TABLE 1.
Strains used in this study
| Strain | Genotype—Plasmid |
|---|---|
| FY406a | MATa(hta1-htb1)Δ∷LEU2 (hta2-htb2)Δ∷TRP1 leu2Δ1 ura3-52 lys2Δ1 lys2-128δ his3Δ200 trp1Δ63—pSAB6 (HTA1-HTB1, URA3) |
| JKY38 | Same as FY406—pJKP18 (HTA1-HTB1, HIS3) |
| JKY41 | Same as FY406—pJKP33 (hta1-S2A-HTB1, HIS3) |
| JKY42 | Same as FY406—pJKP25 (hta1-K5A-HTB1, HIS3) |
| JKY43 | Same as FY406—pJKP26 (hta1-K8A-HTB1, HIS3) |
| JKY44 | Same as FY406—pJKP27 (hta1-S11A-HTB1, HIS3) |
| JKY45 | Same as FY406—pJKP28 (hta1-K14A-HTB1, HIS3) |
| JKY46 | Same as FY406—pJKP29 (hta1-S16A-HTB1, HIS3) |
| JKY47 | Same as FY406—pJKP30 hta1-S18A-HTB1, HIS3) |
| JKY71 | Same as FY406—pJKP34 (hta1-K22Q-HTB1, HIS3) |
| JKY78 | Same as FY406—pJKP67 (hta1-K22R-HTB1, HIS3) |
| FY986a | Same as FY406—pJH161 (hta1Δ4-20-HTB1, HIS3) |
| JKY31 | Same as FY406—pJKP20 (hta1-K120A-HTB1, HIS3) |
| JKY32 | Same as FY406—pJKP21 (hta1-K121A-HTB1, HIS3) |
| JKY33 | Same as FY406—pJKP22 (hta1-S122A-HTB1, HIS3) |
| JKY72 | Same as FY406—pJKP35 (hta1-S122D-HTB1, HIS3) |
| JKY34 | Same as FY406—pJKP23 (hta1-K124A-HTB1, HIS3) |
| JKY35 | Same as FY406—pJKP24 (hta1-T126A-HTB1, HIS3) |
| JKY30 | Same as FY406—pJKP19 (hta1-K127A-HTB1, HIS3) |
| JKY73 | Same as FY406—pJKP36 (hta1-K127Q-HTB1, HIS3) |
| FHY3b | Same as FY406—pJD151 (hta1-S129A-HTB1, HIS3) |
| JKY74 | Same as FY406—pJKP37 (hta1-S122A,K127A-HTB1, HIS3) |
| JKY75 | Same as FY406—pJKP38 (hta1-S122A,S129A-HTB1, HIS3) |
| JKY112c | Same as FY406—p(hta1-T126A,S129A-HTB1, HIS3) |
| JKY79 | Same as FY406—pJKP68 (hta1-K127A,S129A-HTB1, HIS3) |
| JKY80 | Same as FY406—pJKP70 (hta1-S122A,K127A,S129A-HTB1, HIS3) |
| JKY81 | Same as FY406—pJKP69 (hta1-S122A,T126A,S129A-HTB1, HIS3) |
| T69c | Same as FY406—pJH69 (hta1-K120*-HTB1, HIS3) |
| JKY3d | Δho Δhml:ADE1 Δhmr:ADE1 lys5 leu2,3-112 trp1∷hisG ura3-52 ade3∷GAL10:HO Δrad51∷LEU2 |
| JKY5d | Δho Δhml:ADE1 Δhmr:ADE1 lys5 leu2,3-112 trp1∷hisG ura3-52 ade3∷GAL10:HO Δrad52∷TRP1 |
| JKY36 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Δku70∷kanMX |
| JKY121 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Δsod1∷kanMX |
| JKY130 | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Δrad4∷kanMX |
Asterisk indicates stop codon.
d
Moore and Haber (1996).