TABLE 3.

Genomic DNA amplification (DNA), DNA methylation (5mC), and RNA levels (RNA) in Sadhu elements in a set of Arabidopsis accessions

		Sadhu3-1a			Sadhu7-2			Sadhu6-1
Accession	Stock no.	DNA	5mC	RNA	DNA	5mC	RNA	DNA	5mC	RNA
Br-0	CS22628	Yes	Yes	+	No	NT	NT	Yes	Yes	−
Bur-0	CS22656	Yes	Yes	−	No	NT	NT	Yes	Yes	−
Col	LehleWT-2	Yes	Yes	−	Yes	Yes	−	Yes	Yes	−
Cvi	LehleWT-18	Yes	Yes	++	No	NT	NT	No	NT	−
Cvi-0	CS22614	No	NT	NT	No	NT	NT	No	NT	−
Fei-0	CS22645	Yes	Yes	−	No	NT	NT	Yes	Yes	−
Kn-0	CS6762	No	NT	NT	Yes	Yes	−	Yes	Yes	−
Kondara	CS22651	Yes	Yes	−	No	NT	NT	Yes	No	+++
Kz-1	CS22606	No	NT	NT	No	NT	NT	Yes	No	+++
N13	CS22491	Yes	Yes	−	Yes	Yes	−	Yes	Yes	−
No-0	CS6805	No	NT	NT	Yes	Yes	−	No	NT	−
Po-0	CS6839	Yes	Yes	++	No	NT	NT	Yes	No	+++
Pro-0	CS22649	Yes	Yes	−	No	NT	NT	Yes	No	+++
Pu2-7	CS22592	Yes	Yes	−	Yes	Yes	−	Yes	Yes	−
Ra-0	CS22632	Yes	No	+++	Yes	Yes	−	Yes	Yes	−
Tamm-27	CS22605	No	NT	NT	No	NT	NT	Yes	No	−
Ts-1	CS22647	No	NT	NT	No	NT	NT	Yes	No	−
Tsu-1	CS22641	Yes	Yes	−	Yes	Yes	−	Yes	Yes	++
Van-0	CS22627	Yes	Yes	−	No	NT	NT	Yes	Yes	−
Wei-0	CS22622	Yes	Yes	−	Yes	Yes	−	Yes	Yes	−
Ws-2	CS22659	Yes	Yes	−	Yes	Yes	−	Yes	Yes	−

NT, not tested. Genomic DNA amplification uses X1 and Y1 primers (supplemental Table 1 at http://www.genetics.org/supplemental/). DNA methylation is determined by McrBC–PCR using X1 and Y1 primers. RNA levels are detected by RT–PCR: −, none detected; +, low levels; ++, moderate levels; +++, high levels.

^aData are from Rangwala et al. (2006).