TABLE 2.
Synthetic genetic interactions with the not4L35A allele
| ORFa | Name | SL/SSb | Function/processc |
|---|---|---|---|
| YDR069Cde | DOA4 | SL | Protein deubiquitination |
| YDR313C | PIB1 | SL | Protein ubiquitination/E3 ligase activity |
| YFR010W | UBP6 | SS | Protein deubiquitination |
| YGL058We | RAD6 | SS | DNA repair/protein ubiquitination |
| YMR275Ce | BUL1 | SL | Protein ubiquitination |
| YLR285We | NNT1 | SS | Chromatin silencing |
| YNL229C | URE2 | SL | Transcription corepressor activity |
| YNL248Ce | RPA49 | SL | Transcription from Pol I promoter |
| YNL278We | CAF120 | SS | Transcription from RNA pol II promoter |
| YOL051We | MED15 | SS | Transcription from Pol II promoter |
| YOL054We | PSH1 | SL | RNA elongation from Pol II promoter |
| YOL148Ce | SPT20 | SS | Transcription/chromatin modification |
| YOR290Ce | SNF2 | SL | Chromatin remodeling |
| YJR060We | CBF1 | SS | DNA replication |
| YKL213Ce | DOA1 | SS | Double-strand break repair via NHEJ |
| YLR233C | EST1 | SL | Telomere maintenance |
| YLR320We | MMS22 | SS | DNA repair (DSBR) |
| YAL002W | VPS8 | SS | Late endosome to vacuole transport |
| YDL192We | ARF1 | SS | GTPase activity/ER to Golgi transport |
| YLR360W | VPS38 | SL | Late endosome to vacuole transport |
| YNR006We | VPS27 | SL | Protein binding |
| YOR068C | VAM10 | SS | Vacuole fusion |
| YOR332We | VMA4 | SL | Vacuolar ATPase |
| YPL234C | TFP3 | SS | Vacuolar ATPase |
| YPR173Ce | VPS4 | SL | Late endosome to vacuole transport |
| YLR200We | YKE2 | SS | Protein folding/prefoldin complex |
| YKL009Wde | MRT4 | SL | rRNA processing |
| YOR302Wde | SL | Regulation of protein biosynthesis | |
| YLR289We | GUF1 | SL | GTPase activity |
| YBR133Cde | HSL7 | SS | Protein arginine N-methyltransferase |
| YJR104Ce | SOD1 | SS | Superoxide metabolism |
| YDR452W | PHM5 | SL | Polyphosphate metabolism |
| YKL183We | LOT5 | SS | Unknown |
| YKR005C | SL | Unknown | |
| YKR035C | SS | Unknown | |
| YLR232W | SL | Unknown | |
| YPL168W | SS | Unknown |
NHEJ, nonhomologous end joining; DSBR, double-strand break repair.
a
ORFs identified in both not4L35A and not4Δ screens are underlined.
b
Synthetic lethal and synthetic sick, respectively.
c
Annotations derived from the Saccharomyces genome database.
d
These ORFs are frequently identified in SGA screens, even when performed against wild-type query strains (E. Cameroni and C. De Virgilio, unpublished results).
e
Interactions confirmed by random spore analysis and/or tetrad dissection.