TABLE 3.
Synthetic genetic interactions with the not4Δ allele
| ORFa | Name | SL/SSb | Function/Processc |
|---|---|---|---|
| YBR170Cd | NPL4 | SL | ER-associated protein catabolism |
| YDR313C | PIB1 | SL | Protein ubiquitination/E3 ligase activity |
| YFR010W | UBP6 | SS | Protein deubiquitination/26S proteasome |
| YMR275Cd | BUL1 | SL | Protein ubiquitination |
| YPL084Wd | BRO1 | SL | Ubiquitin-dependent protein catabolism |
| YGL025C | MED3 | SS | Transcription from RNA pol II promoter |
| YLR266Wd | BUR2 | SL | CDK/RNA pol II transcription |
| YOL051Wd | MED15 | SL | Transcription from RNA pol II promoter |
| YOL054Wd | PSH1 | SS | RNA elongation from Pol II promoter |
| YPR075Wd | NOT5 | SL | Transcription from RNA pol II promoter |
| YLR320Wd | MMS22 | SS | DNA repair (DSBR) |
| YAL002W | VPS8 | SS | Late endosome to vacuole transport |
| YDL192Wd | ARF1 | SL | ER to Golgi transport |
| YJL188C | BUD19 | SS | Bud site selection |
| YOR069Wd | VPS5 | SL | Retrograde transport |
| YOR332Wd | VMA4 | SL | Vacuolar ATPase |
| YPL234C | TFP3 | SL | Vacuolar ATPase |
| YPR036Wd | VMA13 | SL | Vacuolar ATPase |
| YKR076W | ECM4 | SS | Cell wall organization and biogenesis |
| YLR200Wd | YKE2 | SL | Protein folding/prefoldin complex |
| YOR302W | SS | Protein biosynthesis | |
| YDR417C | SS | Unknown | |
| YJL169W | SS | Unknown | |
| YKL183Wd | LOT5 | SS | Unknown |
| YMR085W | SS | Unknown |
DSBR, double-strand break repair.
a
ORFs identified in both not4L35A and not4Δ screens are underlined.
b
Synthetic lethal and synthetic sick, respectively.
c
Annotations derived from the Saccharomyces genome database.
dInteractions confirmed by random spore analysis and/or tetrad dissection.