TABLE 2.
Crossover distribution in wild-type and Msh6 mutants
Interval | I | II | III | IV | V | VI | VII | VIII | IX | X | XI |
---|---|---|---|---|---|---|---|---|---|---|---|
Size (bp) | 424 | 511 | 269 | 31 | 10 | 376 | 443 | 1057 | 304 | 191 | 164 |
Wild-type COs | 10 | 12 | 7 | 0 | 0 | 8 | 13 | 32 | 8 | 7 | 1 |
Msh6 COs | 9 | 3 | 8 | 0 | 1 | 3 | 3 | 16 | 4 | 2 | 2 |
The 3.8-kb region between the ry606 and ry531 mutant sites was divided into 11 intervals defined by the 10 polymorphisms in this region (Figure 1, supplemental Table S1 at http://www.genetics.org/supplemental/). Intervals are listed in chromosomal order from left (ry606 mutation) to right (ry531 mutation). The exchange positions of 105 COs from wild-type females and the 51 class I COs from Msh6 mutants were determined by PCR and sequencing. The number of COs with an exchange in each interval is given. The distributions are not significantly different from one another (P = 0.37).