Figure 2.—

Functional characterization of basA. (A) Complementation of basA1 mutant. basA1 mutant, a complemented strain cotransformed with a basA PCR product, and plasmid pRG3-AMA1, a basA1 strain transformed with vector pRG3-AMA1 and wild-type strain (GR5), were grown at 42° for 3 days. (B) Restoration of wild-type growth of basA1 mutant with exogenous phytosphingosine on solid medium. Wild-type strain A28 or basA1 mutant conidiophores were inoculated on MAG with PHS at the indicated dosages and incubated at 42° for 3 days. (C) Sensitivity of basA1 mutant to AbA. Strains were streaked onto MAG plates with or without 0.25 μg/ml AbA and incubated at 28° for 3 days.