Figure 4.—

Effects of sphingolipid synthesis disruption on cell wall structure. (A and C) Wild-type strain A28 conidia were germinated at 28° for 12 hr and then shifted to a drug-free YGV (control) or to YGV supplemented with 0.25 μg/ml AbA, 20 μg/ml of myriocin, 1 μg/ml dihydrosphingosine, or 1 μg/ml phytosphingosine and incubated for an additional 1 hr. (B) Wild-type strain A28 and alcA(p)∷lagA strain ASL10 conidia were grown in alcA(p)-repressing medium YGV at 42° for 13 hr. (D) basA1 mutant conidiospores were germinated in YGV at 28° for 12 hr and shifted to fresh YGV with or without 20 μg/ml of myriocin for an additional 1 hr of incubation at 42°. Hyphae were fixed and stained with Calcofluor. Bar, 3 μm.