Table 1.
Forward (F) and reverse (R) primers for amplification of microsatellites in Uta stansburiana
| Primer | Primer sequence (5′ to 3′) | Repeat | Ta, °C | Alleles | Ho |
|---|---|---|---|---|---|
| 10,000 M's-F | AAC-CAT-ACA-GCA-TAG-CAG-TGA-T | (CA)12 | 54 | 11 | 0.30 |
| 10,000 M's-R | AAG-CAT-CAT-GTT-TAC-CAG-ATG-C | ||||
| BRtt-F | CAT-TTC-ATG-CAC-TCT-TGA-TGC-AC | (CA)10 | 51 | 7 | 0.73 |
| BRtt-R | GGG-CAC-AGA-AGA-TGT-TAT-AGA-C | ||||
| IGs-F | GAG-CTA-GGC-AAT-ATG-TAC-TTA-ATG | (CA)14 | 54 | 12 | 0.77 |
| IGs-R | TGT-ACC-ATC-CTG-CAA-CGT-TGT-T | ||||
| MCC-F | TCA-GCT-GTA-ACA-CCC-AGA-AAC | (CA)6A(CA)6 | 53 | 8 | 0.20 |
| MCC-R | TTA-ACT-GCC-AGA-AAA-GGA-CCG | ||||
| NGff-F | GGG-AAT-CAG-GCA-GCA-CAC-AAT | (CA)10 | 58 | 6 | 0.38 |
| NGff-R | TTG-TCA-GCA-AAC-TCC-AGC-GG | ||||
| PLkn-F | GTA-CCT-TGT-GAC-TGC-AGT-GCT | (CA)17 | 58 | 12 | 0.76 |
| PLkn-R | TTG-AGA-CAC-AGG-AGG-CAG-AAG | ||||
| SMcL-F | TAA-TCT-TTG-CAT-ACT-GAG-AT | (CA)15 | 51 | 12 | 0.81 |
| SMcL-R | CCT-AGC-ACA-TCT-CTA-GTA-AG | ||||
| SPhill-F | GAT-CAT-ATA-CTG-GTT-TAA-GAC-A | (CA)9 | 54.5 | 15 | 0.81 |
| SPhill-R | TCA-CAC-ATC-GAC-TCC-AAA-GTC-AG | ||||
| SVeg-F | TGG-TTG-CAA-TGA-GCA-TAT-CAG | (CA)3CG(CA)6 | 51 | 2 | 0.12 |
| SVeg-R | TCA-TTT-CAC-CAT-GCT-AGC-AA |
Also listed are the core repeat (from cloned individual), PCR amplification annealing temperature (Ta), total number of alleles, and observed heterozygosity at each locus.