Figure 3.

Serotype A strain 125.91 contains a novel MATa-specific STE20 allele. Genomic DNA was isolated from three control strains JEC20 (MATa serotype D), JEC21 (MATα serotype D), and H99 (MATα serotype A), and from three clinical isolates from Tanzania (123.91, 124.91, and 125.91). PCR analysis was conducted with primers specific to mating-type- and/or serotype-specific alleles of the STE12α, MFα2, STE20α, and STE20a genes. Under low-stringency conditions, a novel STE20a allele from strain 125.91 was identified and is indicated by an arrow.