Figure 5.

Overexpression of Ste12α induces filament formation in strain 125.91. A 5-FOA-resistant ura5 derivative of strain 125.91 [MATa (A)] was transformed with plasmids containing a galactose-inducible allele of the transcription factor STE12α or the G-protein β-subunit GPB1. Strains were grown on solid filamentation medium containing 0.5% glucose (Glu) or 2% galactose (Gal), and photographed after 5 days of incubation at 30°C at ×10 magnification. In contrast to strain 125.91, overexpression of GPB1 induced filament formation in the serotype D MATa strain JEC34 [MATa (D)].