Figure 4.
Telomerase activity in transduced Strain C and A HFKs. Real-time quantitative TRAP assays were performed on cell extracts from Strain C and A HFKs transduced with either LXSH or TERT and cultured on plastic alone (P), in the presence of feeder cells (F), or transferred from the co-culture environment to plastic alone (F to P). Strain A TERT/Feeders to Plastic #5 cells, transferred form the co-culture environment to plastic at PD 51, are represented above. Approximate PDs at which telomerase activity was measured were Strain C: PD 12 (TERT P), PD 12 (LXSH F), PD 12 (LXSH P), PD >100 (TERT F), PD >100 (TERT F to P); Strain A: PD >100 (TERT F), PD >100 (TERT F–P). Telomerase activity is measured in standard cell equivalents relative to our control cell line. Error bars represent s.d. of the mean.