Figure 8.
Reintroduction of p16 expression causes growth arrest and a senescent morphology in immortalized Strain C and A TERT-transduced HFKs transferred from co-culture with Feeders to Plastic. Late passage (PD>100) Strain C and A TERT Feeders to Plastic cells were infected with retrovirus carrying either pBABE-p16 or pBABE alone. Cells were incubated with BrdU before fixation and stained with antibodies to both p16 and BrdU. DAPI staining was performed for visualization of all HFK nuclei in the microscopic field. Strain A TERT/Feeders to Plastic #5 cells are represented above.