TABLE 1.

Expression of RAG1 paralogs in different Xenopus species

Species (ploidy)	Tissue	Expected paralogs	Observed expressed paralogs	Cloned (C) or directly sequenced (DS)
S. new tetraploid 1 (4x = 40)	Brain	α, β	α, β	C
	Testes	α, β	α, β	DS
	Liver	α, β	α, β	DS
X. laevis (4x = 36)	Bone marrow	α, β	α, β	C, DS
	Heart	α, β	α	DS
	Liver	α, β	α, β	DS
	Spleen	α, β	α, β	DS
	Liver	α, β	α, β	DS
X. gilli (4x = 36)	Bone marrow	α, β	α, β	C
X. borealis (4x = 36)	Brain	α, β	α	DS
	Liver	α, β	α	DS
	Testes	α, β	α	DS
X. muelleri (4x = 36)	Brain	α, β	α	DS
	Testes	α, β	α	DS
	Bone marrow	α, β	α	DS
X. amieti (8x = 72)	Liver	α1, α2, β1, β2	α1, α2a	C
	Brain	α1, α2, β1, β2	α1	DS
X. andrei (8x = 72)	Bone marrow	α1, α2, β1, β2	α1, α2, β1a	C, DS
	Spleen	α1, α2, β1, β2	α1	DS
	Brain	α1, α2, β1, β2	α1	DS
X. new octoploid (8x = 72)	Bone marrow	α1, α2, β1, β2	α1, α2	C
	Brain	α1, α2, β1, β2	α1, α2 (low)	DS
	Heart	α1, α2, β1, β2	α1 (low), α2	DS
	Testes	α1, α2, β1, β2	α1, α2	C
X. boumbaensis (8x = 72)	Bone marrow	α1, α2, β1, β2	α1, α2, β1a	C
	Brain	α1, α2, β1, β2	α1, β1a	DS
	Heart	α1, α2, β1, β2	β1a	DS

Expression was confirmed by amplifying cDNA across an intron and either cloning (C) or directly sequencing (DS) the PCR product.

^aExpressed paralogs that are degenerate.