TABLE 1.
Enhancers and suppressors of Bchs overexpression eye phenotype
| Gene and allele | Modification | Bloomington stock no. | Homologs, protein domains, and functions |
|---|---|---|---|
| Vesicle trafficking | |||
| atg100305 | En | 11484 | Serine/threonine kinase, Tor effector |
| atg600096 | En | 11487 | Beclin-1, mitochondrial protein |
| atg18KG03090 | WEn | 13945 | WD40 domains, binds PIP3-5P2 lipid |
| atg2EP3697 | Su | 17156 | Hydrophilic protein, preautophagic structure |
| atg8aEP362 | Su | 14639 | LC3, lipid attachment and microtubule binding |
| orange49h | En | 2385 | ξ-subunit of AP-3 complex |
| ruby1 | En/Su | 88 | β-subunit of AP-3 complex |
| garnet1 | En/Su | 12 | δ-subunit of AP-3 complex |
| carmine1 | En/Su | 21 | μ-subunit of AP-3 complex |
| spinsterk09905 | En | 10948 | Integral membrane protein |
| deep orange1 | En | 3936 | Vps18p, ubiquitin ligase E3 |
| deep orange4 | En | 35 | Vps18p, ubiquitin ligase E3 |
| deep orange8 | En | 28 | Vps18p, ubiquitin ligase E3 |
| carnation1 | En | 19 | Vps 33, sec1 protein |
| ras oppositeG27 | SEn | 4381 | Rop, munc-18, n-Sec1 |
| syntaxin 1AΔ229 | En | 4379 | SNARE, coiled-coil, vesicle targeting |
| syntaxin 1301470 | En | 11536 | SNARE, coiled-coil, vesicle targeting |
| hook1 | En | 306 | Coiled-coiled, MVB interactions |
| rab1193Bi | SEn | 4158 | Small GTPase |
| rab11j2D1 | SEn | 12148 | Small GTPase |
| lightoid1 | En | 338 | Rab-RP1, a GTPase |
| claret1 | En | 459 | Guanine nucleotide exchange factor (GEF) |
| Cytoskeletal and motor proteins | |||
| dlic2G0190 | En | 11951 | Dynein LC, microtubule-based motor activity |
| dlic2G0065 | En | 11696 | Dynein LC, microtubule-based motor activity |
| btvBG01771 | En | 12589 | Dynein HC, microtubule-based motor activity |
| α-tubulin84B5 | En | 2412 | 84B, cytoskeletal and microtubule constituent |
| α-tubulin84B7 | En | 2237 | 84B, cytoskeletal and microtubule constituent |
| β-tubulin D | WEn | 2451 | 85D, cytoskeletal and microtubule constituent |
| Ubiquitin and SUMO pathway | |||
| smt3k06307 | En | 10419 | SUMO, ubiquitin-like protein |
| lesswrightr05486 | En | 11410 | SUMO-conjugating enzyme |
| uba1s3484 | En | 10461 | E1 ubiquitin-activating enzyme |
| effetemer1 | En | 4656 | UbcD1, ubiquitin-conjugating enzyme |
| CG1490 | En | 14505 | Ubiquitin-specific protease 7 |
Modifier genes that also demonstrate a phenotypic change with a corresponding deletion chromosome from the second and third deficiency kit screens are underlined. En, enhancer; WEn, weak enhancer; Su, suppressor; En/Su, complex modifier; SEn, strong enhancer.