TABLE 1.
Characterization of selected transgenic UAS-YFP-Rab Drosophila strains
| Rab | Annotation symbol | WT construct/line information | DN construct/line information | CA construct/line information |
|---|---|---|---|---|
| Rab1 | 3320 | UAST/01(80C1) | UAST/01(87B8); 04(58B9) | UASp/12a(68C13); 12c(1E4) |
| Rab2 | 3269 | UAST/02(86E18) | UAST/01(42C7); 05(85E1) | UASp/02(61C8); 25(46E6) |
| Rab3 | 7576 | UASp/02(55B9); 05b(95E1) | UASp/04L(25C6) | UASp/11(76D1); 29a(59E3) |
| Rab4 | 4921 | UASp/9(28B1); 32(100A7) | UASp/37(32D2); 46b(72D7) | UASp/13(33C1); 28(76A1) |
| Rab5 | 3664 | UASp/02(94A14); 08b(59F5) | UASp/01(44B8); 02(75B2) | UASp/01a(65A9); 24(24C5) |
| Rab6 | 6601 | UAST/01(57F6) | UAST/01(23F3); 03(92B3) | UASp/05(61C9); 23(3B6) |
| Rab7 | 5915 | UASp/18(96C1); 21(21B2) | UASp/06(79A2); 19(10E6) | UASp/14(100D2); 19(22A1) |
| Rab8 | 8287 | UASp/09(68C2); 45(42E7) | UASp/09(42E1); 12(83B4) | UASp/05(27F4); 10(63F5) |
| Rab9 | 9994 | UASp/13(21B7); 22(79E4) | UASp/04(70D7); 11(33A2) | UASp/10(31F4); 20(61C9) |
| Rab10 | 17060 | UASp/13(34C4); 21(65A9) | UASp/15(23C5); 25a(97D2) | UASp/01(35D1); 27a(86C5) |
| Rab11 | 5771 | UASp/32(89B7) | UASp/06(88F1); 35(59A3) | UASp/24(43C5); 31(61C8) |
| Rab14 | 4212 | UASp/5L(22E1); 12(100D1) | UASp/01(36B2); 02(99F6) | UASp/02(34D1); 07(67C1) |
| Rab18/RP4 | 3129 | UAST/01(82C5) | UAST/02(30B5) 03(87B8) | UASp/02(99A11); 12(37C1) |
| Rab19/RP3 | 7062 | UAST/02(39B4) | UAST/04(70E2); 05(53D14) | UASp/06(62A6); 15(51E2) |
| Rab21 | 17515/40304 | UAST/04(25B1) | UAST/01(99A5); 03(58A3) | UASp/02(53F8); 09(4F2) |
| Rab23 | 2108 | UASp/01(93D4); 02(21C2) | UASp/01(86E11); 02(58E1) | UASp/04(66D12) |
| Rab26 | 7605 | UAST/01(73D5); 05(35D1) | UAST/02(48A3); 03(74D2) | UAST/01(83C4); 04(54E2) |
| Rab27 | 14791 | UASp/01(35D1); 14(86E11) | UASp/02(89A5) | UASp/05(23A3); 07(3C1) |
| Rab30 | 9100 | UASp/10(53C9) | UASp/07(98A7) | UASp/11(39E3) |
| Rab32/RP1 | 8024 | UASp/03(27F1); 11(76D5) | UASp/04(42C8) | UASp/03(85A5); 08(47F8) |
| Rab35 | 9575 | UASp/15(51A2) | UAST/01(23C4); 06(68B1) | UASp/01(95F1); 11(37B13) |
| Rab39 | 12156 | UASp/13(58D4) | UAST/04(37B1); 05(76F1) | UASp/02(5C2); 14(92B3) |
| Rab40 | 1900 | UAST/03(61C1); 05(60F5) | UAST/01(91F4); 04(26D1) | UASp/07(22D1); 13(61B3) |
| RabX1 | 3870 | UASp/10(84F6); 12(27D3) | UAST/01(44B3); 14(62B1) | UASp/02(92B3); 10(25C6) |
| RabX2 | 2885 | UASp/08(92C1) | UASp/02(24A2); 05(67B11) | UASp/10(22B1); 21(84F6) |
| RabX3 | 32670/2532 | UASp/24(94A1); 38(50B6) | UAST/01(47A7); 03(70C10) | UASp/19(98A7) |
| RabX4 | 31118/13638 | UASp/19(94A1) | UAST/02(91F11); 03(23F3) | UASp/09(44B8); 13a(66D10) |
| RabX5 | 7980 | UASp/13b(27D3); 22b(77B4) | UAST/01(47C1) | UASp/10(6C8) |
| RabX6 | 12015 | UASp/03(79A2); 04(39B4) | UAST/01(31F4); 02(82C3) | UASp/13(60A3); 27(73B5) |
| CG9807 | 9807 | UASp/21(chr.2); 25(75E2) | UAST/01(49F11); 03(82D5) | UASp/04(56F11); 14b(96E6) |
| CG32673 | 32673 | UASp/35(97D7); 42(28C4) | UAST/01(57E1); 02(87A2) | UASp/05(80A4); 17(26B2) |
| CG32671 | 32671 | |||
| Rab9D | 32678 |
Only one line from each chromosome (X, II, or III) and a maximal two lines for each gene were selected to send to the stock center. The detailed information is in this table and in supplemental Table 3 at http://www.genetics.org/supplemental/. Columns indicate Drosophila Rab names, CG numbers, the construct that was used to generate the transgenic lines, and the insertion sites identified by inverse PCR. CG32671, CG32673, CG9807, and CG32678 are very similar and our PCR identified CG9807 and CG32673 products.