Figure 2.—

zip1-4LA exhibits defects in nuclear division yet has synapsed chromosomes. (A) Nuclear division assay of WT (NMY274, blue), zip1Δ (NMY364, green), and zip1-4LA (NMY363, red) strains in the BR1919-8B diploid background. The graph shows the percentage of cells having completed one or both nuclear divisions as a function of time. (B) Examples of spread nuclei after 18 hr of sporulation. Zip1 (green), Red1 (red), and DAPI (blue) staining are shown for WT, zip1Δ, and zip1-4LA strains. Bar, 1 μm.