Figure 3.—

Kinetics of SC formation. (A) Nuclear spreads were prepared for ndt80 (NMY539) and ndt80 zip1-4LA (NMY533) BR1919-8B diploid strains harvested at nine different time points after the introduction into sporulation medium. Nuclei were stained for Zip1 (red), tubulin (green), and DAPI (blue) and placed into four categories on the basis of Zip1 staining: no staining, dotty (punctate Zip1 foci), dotty linear (Zip1 foci and some elongated stretches of Zip1), and linear (fully elongated Zip1). Bar, 1 μm. (B) Plots of the percentage of ndt80 (blue) and ndt80 zip1-4LA (red) nuclei in each category as a function of time.