TABLE 1.
Strain | Genotype | Background | |
---|---|---|---|
BR2495a | BR2495 | ||
MY152b | BR2495 but homozygous zip1∷URA3 | BR2495 | |
NMY101 | MY152 plus pRS315-zip1ΔB | BR2495 | |
NMY102 | MY152 plus pRS315-zip1ΔC | BR2495 | |
NMY103 | MY152 plus pRS315-zip1ΔD | BR2495 | |
NMY104 | MY152 plus pRS315-zip1ΔE | BR2495 | |
NMY105 | MY152 plus pRS315-zip1ΔF | BR2495 | |
NMY106 | MY152 plus pRS315-zip1ΔG | BR2495 | |
NMY107 | MY152 plus pRS315-zip1ΔH | BR2495 | |
NMY109 | MY152 plus pRS315-zip1ΔJ | BR2495 | |
NMY111 | MY152 plus pRS315-ZIP1 | BR2495 | |
NMY112 | MY152 plus pRS315-zip1-4LA | BR2495 | |
NMY113 | MY152 plus pRS315 | BR2495 | |
NMY276 | MY152 plus pRS315-zip1-L643A | BR2495 | |
NMY278 | MY152 plus pRS315-zip1-L650A | BR2495 | |
NMY280 | MY152 plus pRS315-zip1-L657A | BR2495 | |
NMY282 | MY152 plus pRS315-zip1-L664A | BR2495 | |
NMY274c | BR1919-8B 2n | ||
NMY363 | NMY274 but homozygous zip1-4LA | BR1919-8B 2n | |
NMY364 | NMY274 but homozygous zip1∷URA3 | BR1919-8B 2n | |
NMY233 | BR1919-8B 2n | ||
NMY533 | NMY274 but homozygous ndt80∷LEU2 zip1-4LA | BR1919-8B 2n | |
NMY539 | NMY274 but homozygous ndt80∷LEU2 | BR1919-8B 2n | |
NMY385 | NMY274 but homozygous spo11∷ADE2 zip1-4LA | BR1919-8B 2n | |
NMY422 | NMY274 but homozygous spo11∷ADE2 | BR1919-8B 2n | |
NMY602 | NMY274 but homozygous rad17∷LEU2 zip1-4LA | BR1919-8B 2n | |
NMY605 | NMY274 but homozygous rad17∷LEU2 zip1∷URA3 | BR1919-8B 2n | |
NMY608 | NMY274 but homozygous rad17∷LEU2 | BR1919-8B 2n | |
NMY611 | NMY274 but homozygous ddc1∷ADE2 zip1-4LA | BR1919-8B 2n | |
NMY614 | NMY274 but homozygous ddc1∷ADE2 zip1∷URA3 | BR1919-8B 2n | |
NMY617 | NMY274 but homozygous ddc1∷ADE2 | BR1919-8B 2n | |
NMY644 | NMY274 but homozygous mec3∷TRP1 zip1-4LA | BR1919-8B 2n | |
NMY647 | NMY274 but homozygous mec3∷TRP1 zip1∷URA3 | BR1919-8B 2n | |
NMY641 | NMY274 but homozygous mec3∷TRP1 | BR1919-8B 2n | |
NMY340 | NMY274 but homozygous swe1∷KanMX4 zip1-4LA | BR1919-8B 2n | |
NMY345 | NMY274 but homozygous swe1∷KanMX4 zip1∷URA3 | BR1919-8B 2n | |
NMY432 | NMY274 but homozygous swe1∷KanMX4 | BR1919-8B 2n | |
NMY650d | BR1919-8B 2n | ||
NMY661 | NMY650 but homozygous pch2∷HphMX4 zip1-4LA | BR1919-8B 2n | |
NMY664 | NMY650 but homozygous pch2∷HphMX4 zip1∷KanMX4 | BR1919-8B 2n | |
NMY654 | NMY650 but homozygous pch2∷HphMX4 | BR1919-8B 2n | |
NMY562 | NMY274 but homozygous swe1∷KanMX4 pch2∷URA3 zip1-4LA | BR1919-8B 2n | |
NMY559 | NMY274 but homozygous swe1∷KanMX4 pch2∷URA3 zip1∷LYS2 | BR1919-8B 2n | |
NMY565 | NMY274 but homozygous swe1∷KanMX4 pch2∷URA3 | BR1919-8B 2n | |
NMY268e | NMY274 but MATα-bearing chromosome III is circular | BR1919-8B 2n | |
NMY270 | BR1919-8B 2n | ||
NMY272 | BR1919-8B 2n | ||
NMY671 | BR1919-8B 2n | ||
NMY672 | NMY268 but homozygous pch2-HphMX4 zip1-KanMX4 | BR1919-8B 2n | |
NMY673 | BR1919-8B 2n | ||
NMY471f | NMY274 but MATα-bearing chromosome III is circular and congenic with | ||
BR1919-8B 2n | |||
NMY472f | NMY274 but MATα-bearing chromosome III is circular and congenic with | ||
BR1919-8B 2n | |||
NMY473f | NMY274 but MATα-bearing chromosome III is circular and congenic with | ||
BR1919-8B 2n | |||
NMY401 | NMY274 but homozygous zip1-ΔC | BR1919-8B 2n | |
NMY402 | NMY274 but homozygous zip1-ΔD | BR1919-8B 2n | |
NMY403 | NMY274 but homozygous zip1-ΔE | BR1919-8B 2n | |
NMY404 | NMY274 but homozygous zip1-ΔF | BR1919-8B 2n | |
NMY405 | NMY274 but homozygous zip1-ΔJ | BR1919-8B 2n | |
NMY461g | SK1 | ||
NMY462g | SK1 | ||
NMY463g | SK1 |
BR2495 was described by Rockmill and Roeder (1990). The MATα parent of BR2495 is BR1919-8B (Rockmill and Roeder 1990).
MY152 was described by Sym and Roeder (1995).
NMY274 is a diploid strain whose MATα haploid parent is isogenic with BR1919-8B (Rockmill and Roeder 1990) and whose MATa haploid parent was generated by switching the mating type of BR1919-8B (Rockmill et al. 1995).
NMY650 is isogenic with NMY274 except for the markers noted, including a copy of ADE2 inserted at an ectopic location on chromosome III (designated iADE2).
NMY268 is a diploid consisting of a MATα strain isogenic with BR1919-8B that carries a circular version of chromosome III and is spo13∷URA3 mated to MATa BR1919-8B.
NMY471–NMY473 are diploids consisting of a MATα strain congenic with BR1919-8B that carries a circular version of chromosome III and is swe1∷KanMX4 zip1∷LEU2 mated to MATa BR1919-8B strains that are swe1∷KanMX4 and ZIP1 (NMY471) or zip1-4LA (NMY472) or zip1∷URA3 (NMY473).
NMY461–NMY463 are isogenic with SK1 (Alani et al. 1987).