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Heterokaryon assay of tRNA retrograde movement
| Cross | Media | PMI | All nuclei accumulate tRNAGlu-D (%) | Some nuclei accumulate tRNAGlu-D (%) | No nuclei accumulate tRNAGlu-D (%) |
|---|---|---|---|---|---|
| Wild type | SC | 2 | 0 (0) | 1 (1.2) | 83 (98.8) |
| Wild type | SC–Pi | 1 | 81 (83.5) | 14 (14.4) | 2 (2.1) |
| Wild type | SC–Pi | 2 | 64 (72.7) | 9 (10.2) | 15 (17.0) |
| 88Δ | SC | 2 | 79 (95.2) | 4 (4.8) | 0 (0) |
| 88Δ | YEPD | 2 | 36 (97.3) | 2 (2.7) | 0 (0) |
PMI, postmating incubation time.
