TABLE 2.
Fatty acid composition of lipid fractions from wild-type and fat-6;fat-7 double mutants
| Fatty acid | Wild-type TAG | fat-6;fat-7 TAG | Wild type PL | fat-6;fat-7 PL |
|---|---|---|---|---|
| 14:0 | 2.3 ± 0.3 | 2.4 ± 0.6 | 0.7 ± 0.1 | 0.5 ± 0.1 |
| 16:0 | 4.0 ± 2.5 | 2.2 ± 1.9 | 4.1 ± 0.5 | 1.8 ± 1.3* |
| 18:0 | 3.6 ± 0.1 | 20.2 ± 3.1** | 9.3 ± 1.8 | 23.5 ± 4.0* |
| Total saturated | 9.9 | 24.8 | 14.1 | 25.8 |
| 16:1 | 2.3 ± 0.2 | 3.2 | 0.9 ± 0.1 | 3.0 ± 0.1*** |
| 18:1Δ9 | 6.0 ± 0.1 | —*** | 2.2 ± 0.2 | —*** |
| 18:1Δ11 | 14.6 ± 2.6 | 16.8 ± 0.9 | 15.0 ± 1.7 | 26.0 ± 3.9* |
| Total MUFA | 21.8 | 20.0 | 16.8 | 29.0 |
| 18:2 | 3.0 ± 0.6 | —* | 4.6 ± 0.5 | —*** |
| 20:3 | 2.5 ± 0.3 | —** | 6.8 ± 0.5 | —*** |
| 20:4n-6 | 0.8 ± 0.4 | — | 2.5 ± 0.4 | —* |
| 20:4n-3 | 1.5 ± 0.3 | —* | 9.4 ± 0.1 | —*** |
| 20:5 | 3.1 ± 0.5 | —** | 22.0 ± 1.1 | —*** |
| Total PUFA | 11.5 | — | 46.1 | — |
| 18:1 Δ13 | — | 2.2 ± 0.2** | — | 1.7 ± 0.1*** |
| 18:3 Δ8,11,14 | — | 0.8 ± 0.7 | — | 8.3 ± 0.9*** |
| 18:4 Δ5,8,11,14 | — | 0.5 ± 0.5 | — | 5.6 ± 0.8** |
| 18:4 Δ8,11,14,17 | — | 0.5 ± 0.5 | — | 6.3 ± 1.3*** |
| Total unusual | — | 4.0 | — | 21.9 |
| C15iso | 5.7 ± 0.2 | 2.0 ± 0.5** | 1.8 ± 0.1 | —*** |
| C17iso | 3.2 ± 0.2 | 2.1 ± 0.4* | 2.6 ± 0.1 | 0.9 ± 0.3** |
| Total branched | 8.9 | 4.1 | 4.5 | 0.9 |
| 17Δ | 28.8 ± 0.6 | 31.1 ± 2.4 | 6.7 ± 0.7 | 10.6 ± 1.7* |
| 19Δ | 16.8 ± 1.2 | 12.2 ± 1.5* | 6.8 ± 0.5 | 7.7 ± 2.9 |
| Total cyclopropane | 45.6 | 43.3 | 13.5 | 18.3 |
Data are weight percentages of total fatty acids (mean ± SD) of lipid extractions from three independently grown nematode populations measured by gas chromatography. 17Δ, 9,19-methylenehexadecanoic acid; 19Δ, 11,12-methyleneoctadecanoic acid; TAG, triacylglyceride fraction; PL, phospholipid fraction. Dashes indicate fatty acids <0.5%. Values determined to be significantly different from wild-type lipid fractions using an unpaired t-test are *P < 0.05, **P < 0.001, and ***P < 0.0001.