TABLE 1.
Germ-cell migration defects
| Germ cells lost |
|||
|---|---|---|---|
| 0–4 (%) | 5–6 (%) | 7+ (%) | |
| A. Embryos maternally compromised for ttv activity display varying degrees of germ-cell migration defectsa | |||
| ttv germline clone (N = 48) | 58 | 15 | 27 |
| Wild type (N = 49) | 92 | 4 | 4 |
| B. Expression of PtcΔloop2 in the germline disrupts germ-cell migrationb | |||
| nos-GAL4 (N = 122) | 91 | 6 | 2 |
| nos-GAL4; UAS-ptcΔloop2 (N = 100) | 69 | 17 | 14 |
a
Embryos obtained by mating females carrying ttv germline clones with wild type males were stained with anti-Vasa antibody and classified based on the number of germ cells that failed to coalesce with the somatic gonadal precursor cells.
b
Females carrying nos-GAL4 were mated with UAS ptc Δloop2 males (X-linked transgene). In this scheme, female embryos received both the drive and the UAS transgene while male embryos received only the nos-GAL4 driver and consequently serve as an internal control. Embryos were co-immunostained with anti-Sxl and anti-Vasa antibodies. Germ-cell loss in Sxl-positive (female) embryos is considerably higher than that for Sxl-negative (male) embryos.