Figure 4.—

Transcription of MTD1 during gametogenesis. (A) Total RNA samples were prepared as in Figure 2B. Ten micrograms of total RNA were loaded in each lane. A membrane was probed with MTD1, stripped, and reprobed with SAD1, GSM1, and L27a, sequentially. SAD1 encodes minus agglutinin; GSM1 encodes a homeodomain protein expressed in minus gametes. L27a, encoding a 60S ribosomal protein, serves as a loading control. (B) RT–PCR results of MTD1 and two nitrogen-starved gametogenesis (NSG) genes, NSG6 and NSG17. mRNA samples were prepared as in Figure 2B. The top band in the MTD1 lanes derives from contaminating genomic DNA. Mating efficiencies of cells when samples were collected are standardized using mating efficiencies of wild-type tester cells.