Figure 9.—

Co-expression of UAS-ttm50 and UAS-p35 driven by en-GAL4 increases cell density (A–D) and proliferation (E and F) in the imaginal discs. (A) Wing disc nuclei stained by DAPI. (B) en-GAL4-driven gene expression in the posterior compartment marked by UAS-GFP. (C) Tissue morphology visualized by actin stained with Alexa Fluor 647-conjugated phalloidin shows a change in the posterior compartment. (D) Merged image of A–C. (E) en-GAL4-driven gene expression in the posterior compartments of two imaginal discs marked by UAS-GFP. (F) S-phase cells visualized by Brd-U incorporation increased in the posterior compartments. (G) Expression of UAS-GFP driven by ptc-GAL along the anterior posterior boundry of the wing imaginal disc pouch. (H) Increase in Mitotracker Red staining induced by overexpression of UAS-ttm50 driven by ptc-GAL in the same sample.