Figure 1.—

Molecular identification of the levy gene. Schematic of DNA encompassing the levy gene. (A) The levy gene was first localized to genetic position 2-103 using recombination mapping of the levy¹ mutation with visible markers L² and Pin¹ on the right arm of chromosome 2. Deficiency mapping localized the levy gene cytologically between bands 59D11 and 59E. Site-specific recombination mapping in males localized the levy gene between two P-element insertion sites, chrw^k06908 and Dcp-1^k05606 (represented by triangles). (B) Results of molecular analysis of three levy deficiencies (levy², levy³, and levy⁴) generated by P-element mobilization in Dcp-1^k05606 flies suggested that the levy gene is CG17280, pita, or Dcp-1. Gaps in solid lines represent DNA deleted in these three levy deficiency strains. (C) The relative locations of genes in this region are shown as lines. The CG17280 gene was identified as levy by transforming a 2-kb DNA fragment encompassing CG17280 (represented by a line in D) into the germ line of levy¹ mutants. These levy transformants did not exhibit temperature-induced paralytic behavior. The bar at the bottom represents 1 kb of DNA in B–D.