TABLE 2.
Molecular diversity and outcrossing rates in the sampled C. elegans populations
| Genetic diversity |
Outcrossing |
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Inbred? | N | Pairwise difference | H | SD | Θhom | SD | No. of heterozygotes | Observed heterozygosity | f | C.I. | s | C.I. |
| Bar-1004 | h | 15 | 1.251 | 0.699 | 0.084 | 1.774 | 0.739 | 0 | 0.000 | 1.000 | 1.000 | ||
| Bar-0805 | h | 14 | 2.152 | 0.605 | 0.052 | 1.151 | 0.257 | 1 | 0.067 | 0.940 | (0.877–1) | 0.969 | (0.934–1) |
| Bla-0802 | i | 13 | 0.282 | 0.271 | 0.099 | 0.277 | 0.138 | ||||||
| Bla-1105 | h | 24 | 3.437 | 0.832 | 0.046 | 4.014 | 1.432 | 5 | 0.208 | 0.858 | (0.819–0.901) | 0.924 | (0.901–0.948) |
| Fra-1102 | i | 12 | 1.621 | 0.507 | 0.093 | 0.769 | 0.289 | ||||||
| Fra-1004 | h | 12 | 1.522 | 0.754 | 0.058 | 2.377 | 0.793 | 0 | 0.000 | 1.000 | 1.000 | ||
| Fra-1204 | i | 12 | 1.621 | 0.797 | 0.067 | 3.110 | 1.394 | ||||||
| Fra-0205 | i | 12 | 1.712 | 0.609 | 0.087 | 1.171 | 0.438 | ||||||
| Fra-0405 | h | 6 | 1.818 | 0.727 | 0.109 | 2.054 | 1.190 | 0 | 0.000 | 1.000 | 1.000 | ||
| Fra-0505 | h | 12 | 1.779 | 0.757 | 0.060 | 2.427 | 0.846 | 2 | 0.167 | 0.865 | (0.755–1) | 0.927 | (0.86–1) |
| Fra-0605 | h | 82 | 1.003 | 0.533 | 0.045 | 0.852 | 0.156 | 1 | 0.012 | 0.988 | (0.969–1) | 0.994 | (0.984–1) |
| Fra-0705 | i | 12 | 1.152 | 0.522 | 0.099 | 0.815 | 0.327 | ||||||
| Fra-0905 | h | 18 | 1.594 | 0.679 | 0.024 | 1.613 | 0.185 | 0 | 0.000 | 1.000 | 1.000 | ||
| Fra-0106 | h | 57 | 0.896 | 0.504 | 0.051 | 0.760 | 0.158 | 0 | 0.000 | 1.000 | 1.000 | ||
| HerC-0902 | i | 12 | 3.758 | 0.812 | 0.040 | 3.436 | 0.979 | ||||||
| HerC-1105 | h | 19 | 2.504 | 0.762 | 0.068 | 2.502 | 1.002 | 0 | 0.000 | 1.000 | 1.000 | ||
| HerF-1005 | h | 45 | 0.261 | 0.170 | 0.053 | 0.153 | 0.058 | 0 | 0.000 | 1.000 | 1.000 | ||
| HerF-1105 | h | 11 | 0.000 | 0 | 0.000 | ||||||||
| Lis8-0705 | h | 9 | 1.098 | 0.680 | 0.108 | 1.616 | 0.839 | 0 | 0.000 | 1.000 | |||
| Lis12-0705 | h | 20 | 3.103 | 0.836 | 0.042 | 4.125 | 1.386 | 0 | 0.000 | 1.000 | |||
| Lis14-0705 | h | 9 | 0.993 | 0.392 | 0.133 | 0.480 | 0.268 | 0 | 0.000 | 1.000 | 1.000 | ||
| Mer1-0902 | i | 11 | 0.000 | ||||||||||
| Mer1-1005 | h | 18 | 0.324 | 0.210 | 0.088 | 0.198 | 0.104 | 0 | 0.000 | 1.000 | 1.000 | ||
| Mer2-0902 | i | 4 | 0.000 | ||||||||||
| Mer3-0902 | i | 4 | 0.000 | ||||||||||
| Mer3-1005 | h | 1 | 0.000 | 0 | 0.000 | ||||||||
| Mer4-1005 | h | 1 | 0.000 | 0 | 0.000 | ||||||||
| Mer5-1005 | h | 4 | 2.714 | 0.857 | 0.082 | 4.935 | 3.621 | 0 | 0.000 | 1.000 | 1.000 | ||
| Obe-1005 | h | 18 | 0.979 | 0.884 | 0.034 | 6.428 | 2.350 | 1 | 0.056 | 0.973 | (0.92–1) | 0.986 | (0.958–1) |
| Per-0604 | h | 10 | 0.000 | 0 | 0.000 | 1.000 | 1.000 | ||||||
| Per-1004 | h | 12 | 0.464 | 0.304 | 0.115 | 0.326 | 0.176 | 0 | 0.000 | 1.000 | 1.000 | ||
| Per-1204 | i | 9 | 2.028 | 0.758 | 0.077 | 2.440 | 1.100 | ||||||
| Per-0205 | i | 12 | 0.000 | ||||||||||
| Per-0605 | h | 6 | 0.303 | 0.303 | 0.148 | 0.324 | 0.226 | 0 | 0.000 | 1.000 | 1.000 | ||
| Per-0705 | i | 10 | 0.556 | 0.779 | 0.061 | 2.766 | 1.056 | ||||||
| Per-0905 | h | 44 | 0.000 | 0 | 0.000 | ||||||||
| Per-1205 | h | 13 | 1.329 | 0.394 | 0.107 | 0.484 | 0.218 | 0 | 0.000 | 1.000 | 1.000 | ||
| Pri-1004 | h | 35 | 1.559 | 0.601 | 0.069 | 1.134 | 0.335 | 0 | 0.000 | 1.000 | 1.000 | ||
| Pri-0805 | h | 11 | 2.615 | 0.537 | 0.090 | 0.867 | 0.320 | 0 | 0.000 | 1.000 | 1.000 | ||
“Inbred?,” whether individual genotypes were scored after laboratory inbreeding (“i,” inbred strains) or without (“h,” potentially heterozygous); “N,” number of genotyped individuals; “Pairwise difference,” mean number of different pairwise microsatellite loci; “H,” gene diversity (calculated with Arlequin; Excoffier et al. 2005) and its standard deviation (SD); “Θhom,” Θ-parameter calculated after homozygosity and its standard deviation; f, equilibrium inbreeding coefficient (estimator of FIS after Weir and Cockerham 1984, calculated with GDA; Lewis and Zaykin 2001) and its 95% confidence interval (C.I.); s, selfing rate calculated after f and its 95% confidence interval.