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Quantification of the relative expression of the 23 genes selected as being up-regulated in stage X embryo compared to Hamburg and Hamilton (HH) stage 3. Quantitative RT-PCR was conducted at stage X (0 h), HH stage 3 (12 h), HH stage 6 (24 h), and HH stage 9 (30 h) embryos. The relative quantification of gene expression was analyzed by the 2-ΔΔCt method. NA is not annotated from NetAffx analysis tool.
