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. Author manuscript; available in PMC: 2008 Apr 1.

Published in final edited form as: Osteoarthritis Cartilage. 2006 Nov 27;15(4):421–430. doi: 10.1016/j.joca.2006.10.004

Immunohistochemistry of chondrocytes cultured in alginate beads and treated with growth factors. 4a, normal chondrocytes; 4b, OA chondrocytes. A–D, chondrocytes stained with anti-aggrecan antibody (Aggr); E–H, chondrocytes stained with anti-type VI collagen antibody (ColVI); I–L, chondrocytes stained with anti-decorin antibody (Dec); M–P chondrocytes stained with anti-fibronectin antibody (Fbrn). A,E,I,M, mini-ITS control; B,F,J,N, cells treated with IGF-1; C,G,K,O, cells treated with OP-1; D,H,L,P, cells treated with combined OP-1 + IGF-1. Aggrecan and decorin immunostaining was developed with ImmunoPure ABC Alkaline Phosphatase staining kits that produced blue color; type VI collagen and fibronectin stains were developed by the application of horseradish-peroxidase conjugated secondary antibodies that produced brown color. Original magnification 400x.

Immunohistochemistry of chondrocytes cultured in alginate beads and treated with growth factors. 4a, normal chondrocytes; 4b, OA chondrocytes. A–D, chondrocytes stained with anti-aggrecan antibody (Aggr); E–H, chondrocytes stained with anti-type VI collagen antibody (ColVI); I–L, chondrocytes stained with anti-decorin antibody (Dec); M–P chondrocytes stained with anti-fibronectin antibody (Fbrn). A,E,I,M, mini-ITS control; B,F,J,N, cells treated with IGF-1; C,G,K,O, cells treated with OP-1; D,H,L,P, cells treated with combined OP-1 + IGF-1. Aggrecan and decorin immunostaining was developed with ImmunoPure ABC Alkaline Phosphatase staining kits that produced blue color; type VI collagen and fibronectin stains were developed by the application of horseradish-peroxidase conjugated secondary antibodies that produced brown color. Original magnification 400x.