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. 2007 May 24;26(12):2856–2867. doi: 10.1038/sj.emboj.7601723

Figure 5.

Figure 5

Activation of ERK1/2 pathway is necessary and sufficient to promote the release of BimEL from Mcl-1. (A) Cycling CCl39 cells (CM) were serum starved (SF) for 6 h to induce formation of BimEL/Mcl-1 complexes. Cells were then stimulated for 15 min with 10 nM thrombin, with or without 20 μM U0126 or 30 μM LY294002. Whole-cell extracts (input) were used for immunoprecipitation of Mcl-1 and samples were immunoblotted with the indicated antibodies. (B) CR1-11 cells (CCl39 cells expressing ΔRaf-1:ER*) in complete medium (CM) were serum starved (SF) for 6 h to induce formation of BimEL/Mcl-1 complexes. Cells were then stimulated for 1 h with 100 nM 4-HT±20 μM U0126. Whole-cell extracts (input) were used for immunoprecipitation of Mcl-1 and samples were then immunoblotted with the indicated antibodies. Results are taken from a single experiment representative of three.