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. 2007 May 24;26(12):2843–2855. doi: 10.1038/sj.emboj.7601724

Figure 7.

Figure 7

Src-binding domain is essential for p140Cap role in biological processes. (A) Left panel, a schematic representation of full-length p140Cap protein, p140Pro and p140Delta mutants. Right panel, cell extracts of MCF7p140/P9, p140Pro and p140Delta were analysed by Western blot using Myc antibodies. The same filter was re-probed with Src-specific antibodies. (B) Extracts of HEK293 cells transiently transfected with p140FL, p140Pro and p140Delta were immunoprecipitated with Src antibodies. The immunoprecipitate were analysed by Western blot with Myc and Src antibodies. (C) The histogram represents the mean cell area for MCF7-Mock, p140/P9, p140Pro and p140Delta cells plated on FN for the indicated times, calculated as described in Figure 1C. (D) Left panel, the same cells as in (C) were induced to migrate and to invade as described in Figure 3C. (E) Extracts of MCF7-Mock and p140Delta cells plated on FN for 30 min or kept in suspension (S) were tested for in vitro Src kinase assay as shown in Figure 2A (left panel) or for Rac activation as shown in Figure 2B. The results are representative of three independent experiments (*P<0.05).