Table 1.

qRT–PCR analysis of CREB-target genes using three independent MEF isolates of each genotype (n=8–9)

Symbol (mouse)	Name	Δflox EtOH signal (mean±s.d.)	Triple-KIX Δflox EtOH signal (mean±s.d.)	Δflox FI signal (mean±s.d.)	Triple-KIX Δflox FI signal (mean±s.d.)	Ratio of mutant FI (AVE) to control FI (AVE) signals	One-tailed t-test of FI signals
Areg	Amphiregulin	0.02±0.01	0.003±0.002	1.92±0.81	0.12±0.06	0.063	<0.0001
Btg2	B-cell translocation gene 2, antiproliferative	5.43±2.16	4.40±1.00	35.35±13.04	25.31±17.78	0.72	0.11
Crem ICER	cAMP-responsive element modulator, ICER isoform	0.36±0.12	0.40±0.18	7.98±2.05	8.40±3.87	1.05	0.39
Dusp1	Dual specificity phosphatase 1	9.45±4.52	4.68±1.76	103.00±28.61	109.30±41.23	1.06	0.35
Hlf	Hepatic leukemia factor	0.08±0.08	0.03±0.03	2.17±1.52	0.69±0.55	0.32	0.007
Junb	Jun-B oncogene	5.03±2.57	4.76±1.90	59.72±21.79	74.80±57.09	1.25	0.23
Nr4a3	Nuclear receptor subfamily 4, group A, member 3	0.04±0.01	0.03±0.01	40.49±7.87	29.16±22.41	0.72	0.09
Rgs2	Regulator of G-protein signaling 2	1.23±0.62	1.79±0.70	64.97±18.55	46.25±29.27	0.71	0.06
Relative expression (divided by 1000) set to the lowest real-time PCR value for this data set of eight genes. Test gene signal normalized to Actb mRNA. Note that the mean of the ratios of mutant to control FI signals is 0.74 for these eight genes, similar to the global average seen in the Affymetrix experiment in Figure 1.