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. 2000 Dec 19;97(26):14500–14505. doi: 10.1073/pnas.97.26.14500

Figure 1.

Figure 1

(A) A well-defined interval on chromosome III in which a 1.8-kb LEU2 gene is flanked closely by URA3 and ADE1 genes. The positions of leu2-K and leu2-R are shown by o and x, respectively. (B) Recombination in strain WYL 191 is largely confined to the 1.8-kb interval containing the 2-kb LEU2 gene bounded between flanking heterologies, a 20-kb deletion (URA3) and a 2.2-kb insertion (ADE1). (C) A 117-bp HO cleavage site (leu2-cs, arrow) was inserted into the LEU2 gene at Asp718. Sites of Spo11p cleavage are located approximately 400 bp centromere-distal to leu2-cs. The opposite homologue carries leu2-R. All strains expressing HO contain mutations at MAT that prevent HO cleavage. (D) DAM454 and DAM457 carry leu2-cs and a wild-type LEU2 allele. (E) DAM530 has the same chromosome III structure as that shown in D, except that the HML locus on the leu2-cs-containing chromosome was disrupted by the insertion of URA.