Table 1.
Spores with no crossing over | Spores with crossing over | |||||||
---|---|---|---|---|---|---|---|---|
A. Analysis of random spores from WYL237 bearing no heterologies around LEU2 (Fig. 1A) | ||||||||
Random spores | His−Ura+Ade− | His−Ura−Ade+ | Total | His−Ura+Ade+ | His−Ura−Ade− | Total | Total | % crossing over |
Leu− | 84 | 99 | 183 | 28 | 23 | 31 | 214 | 14 |
Leu+ | 17 | 81 | 98 | 127 | 14 | 141 | 239 | 60 |
B. Analysis of random spores from WYL191 containing heterologies flanking LEU2 (Fig. 1B) | ||||||||
Random spores | His+Ura−Ade− | His−Ura+Ade+ | Total | His+Ura−Ade+ | His−Ura+Ade− | Total | Total | % crossing over |
Leu− | 379 | 331 | 710 | 33 | 23 | 56 | 766 | 7 |
Leu+ | 50 | 245 | 295 | 457 | 45 | 502 | 797 | 63 |
C. Analysis of Leu+ random spores from strains with and without SPO13∷HO (Fig. 1C) | ||||||||
Strain | His+Ura−Ade− | His−Ura+Ade+ | Total | His+Ura−Ade+ | His−Ura+Ade− | Total | Total | % crossing over |
DAM528 | 144 | 8 | 152 | 28 | 115 | 143 | 295 | 49 |
SPO11 | ||||||||
DAM509 | 145 | 4 | 149 | 7 | 119 | 126 | 275 | 46 |
SPO11 | ||||||||
SPO13∷HO |