Figure 2. Effect of lsr2 Gene Inactivation and Overexpression on Activity of the M. tuberculosis iniBAC Promoter.
The activity of the PiniBAC in the presence or absence of 5 ug/ml EMB is indicated by β-galactosidase units.
(A) “Uninduced” cultures without EMB treatment.
(B) “Induced” cultures treated with EMB.
Open columns, β-galactosidase activity of an NJS20.w control containing a random transposon insertion; dashed columns, the lsr2 transposon mutant strain NJS20.1; closed columns, the complemented mutant NJS20.1c. The mean and standard deviations of at least triplicate experiments of each strain are shown. Note that different scales were used for β-lactamase units in (A) and (B).