Figure 6. Two-Dimensional TLC Analysis of the Polar Lipids Extracted from M. smegmatis Strains.

(A) Two-dimensional TLC of polar lipid fractions from wild-type M. smegmatis Mc²155, lsr2 transposon mutant NJS20.1, and complemented strain NJS20.1c. The solvent system is as follows: first dimension, chloroform/methanol/water (60/30/6); second dimension, chloroform/acetic acid/methanol/water (40/25/3/6). The spots were visualized with orcinol. The black arrow marks the spot present in the NJS20.1 mutant and not in either the control or the complemented strain.

(B) The cells were grown to log phase and labeled with [1-¹⁴C]-acetate. The black arrow indicates the new lipid.