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. 2007 Jun 22;3(6):e87. doi: 10.1371/journal.ppat.0030087

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© 2007 Colangeli et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The pGEM plasmid was pre-incubated with different concentrations of Lsr2 where indicated. In vitro transcription was then performed either in transcription buffer or in buffer plus added imidazole (the Lsr2 elution) at the appropriate control concentration. Lane 1, pGEM with 200 ng of Lsr2; lane 2, pGEM with 600 ng of Lsr2; lane 3, pGEM with 40 nM imidazole (control for lane 1); lane 4, pGEM with 120 nM imidazole (control for lane 2).