Figure 5.

HCMV mRNA accumulation after infection with wt or mutant viruses at a relatively low multiplicity. (A) Human fibroblasts were infected with wt or ADsubUL82^−UL82 virus (0.1 pfu per cell). Cell lysates were prepared at the indicated times after infection and assayed for IE1, IE2, and pp28 protein expression by Western blotting. (B) Human fibroblasts were infected with wt or ADsubUL82^−UL82 virus at a multiplicity of 0.1 pfu per cell. At 144 h after infection, RNA was isolated and reverse transcribed in the presence of [³²P]dCTP. The labeled cDNA was then used to probe HCMV gene arrays. Actin was used to confirm that equal quantities of each sample were analyzed, as described in the legend for Fig. 3A.