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. Author manuscript; available in PMC: 2007 Jun 21.
Published in final edited form as: J Immunother. 2007 Apr;30(3):261–273. doi: 10.1097/01.cji.0000211339.81211.25

FIGURE 3.

FIGURE 3

aCTL adhesion to glioma-derived ECM proteins and conjugate formation between aCTL and glioma cells. A, The adhesion of aCTL to IR clone-derived ECM proteins are expressed as a percentage ± SEM of the aCTL adhered to parental ECM proteins, as derived from absorbance measurements. B, Confocal micrograph demonstrates conjugates of adherent CFSE-labeled 13-06-IR29 glioma cells with CellTracker Orange-labeled aCTL. Multiple smaller sized aCTL (arrows) are associated with the larger individual 13-06-IR29 cells. Damage to the glioma cells is readily visualized by the membrane blebbing (arrowheads). Bar = 20 μm. C, Percentages of parental glioma cells and IR clones bound to aCTL ± SEM, as determined by confocal microscopic examination of 5 random 20 × fields for each glioma cell type incubated with aCTL for 30 minutes.