Parental glioma cells and IR clones are sensitive to calcium-dependent lysis by aCTL. Parental 13-06-MG cells and IR clones, 13-06-IR29 and 13-06-IR30, were coincubated with anti-13-06 aCTL at a 12.5:1 E:T ratio for 4 hours. In some wells the calcium-chelator, EGTA, was added to inhibit perforin and granzyme-mediated cell lysis. The percentages of glioma cells lysed ± SEM, obtained in the presence or absence of EGTA, are shown.