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Expression of PLC-β1 and PLC-γ1 in adhesion-competent trophoblast cells. Blastocysts cultured to GD 7 were fixed, permeabilized, and labeled by immunofluorescence using non-immune IgG (IgG) or antibodies against PLC-γ1 or PLC-β1, as detailed in the Materials & Methods. Representative 1 μm optical sections are shown for antigen visualized using Texas-Red-conjugated secondary antibody and scanning laser confocal microscopy.
