Table 1.

Diapause upregulated genes from Cx. pipiens, isolated by suppressive subtractive hybridization. Percent identities, organisms, and accession nos. were retrieved from GenBank (http://www.ncbi.nlm.nih.gov/BLAST/). In the blast column, “n” and “x” indicate putative identities obtained through a nucleotide (BLASTn) and/or translation (BLASTx) query, respectively. Confirmation by northern blot is indicated by an “X” in early (18°C, short daylength; 7–10 days post adult eclosion) and late (18°C, short daylength; 56–59 days post adult eclosion) diapause, ED and LD, respectively.

						northern
clone	size	acc. no.	putative identity	blast	% identity, organism, accession no.	ED	LD
regulatory
CpiLD-A38	469	DQ401432	ribosomal protein S3A	n	91%, Aedes aegypti, AY431319		X
CpiED-B03	356	DQ401433	putative transcription elongation factor B polypeptide 3 binding protein 1	x	40%, Mus musculus, XP_143238
CpiLD-L01	296	DQ401434	putative ribosomal protein S6	n	97%, Aedes aegypti, AY431732		X
CpiED-M01	70	DQ401435	putative methoprene-tolerant protein	n	100%, Anopheles gambiae, DQ303468
CpiLD-C04	295	DQ401436	ribosomal protein S24	n	98%, Aedes aegypti, AY064700		X
food utilization
CpiED-A47	230	AY958428	fatty acid synthase	n	85%, Armigeres subalbatus, AY441061	X
stress response
CpiED-A24	306	DQ401437	selenoprotein	n	87%, Anopheles gambiae, AF457547
CpiLD-A06	1,515	DQ401438	putative aldehyde oxidase	n	93%, Culex pipiens quinquefasciatus, AF202953		X
CpiLD-A11	483	DQ401439	small heat shock protein	x	49%, Sarcophaga crassipalpis, AAC63387		X
Cytoskeletal
CpiED-A09	408	DQ322244	Actin	n	93%, Culex pipiens pipiens, DQ023309	X
metabolic function
CpiED-A07	116	DQ401440	putative mitochondrial malate dehydrogenase	n	92%, Aedes aegypti, AY433139	X
CpiLD-E09	1,055	DQ401441	putative methylmalonate-semialdehyde dehydrogenase	x	88%, Drosophila melanogaster, Q7KW39		X
CpiLD-H04	805	DQ360493	cytochrome c oxidase subunit III	n	87%, Aedes aegypti, AY431617	X	X
CpiLD-H06	912	DQ360492	cytochrome c oxidase subunit I	n	92%, Culex tarsalis, AF425847	X
ribosomal
CpiED-A32	177	DQ401442	ribosomal protein L18	n	86%, Aedes aegypti, AY431545	X
CpiED-E08	105	DQ401443	ribosomal protein 27A	x	100%, Aedes aegypti, AY432783
CpiLD-A01	231	DQ401444	large ribosomal subunit RNA	n	98%, Aedes aegypti, AY431935	X
CpiLD-C02	324	DQ401445	Wolbachia 23S ribosomal RNA, endosymbiont of Cx. pipiens pipiens	n	97%, Wolbachia pipientis, U23710		X
CpiLD-H09	291	DQ401446	28S large subunit ribosomal RNA	n	87%, Uranotaenia lowii, AF417833
transposable elements
CpiLD-D02	897	DQ401447	putative reverse transcriptase	x	43%, Aedes aegypti, CAI96711		X
CpiLD-D11	889	DQ401448	similar to miniture inverted-repeat transposable element (Mimo family)	n	90%, Culex pipiens, AF217612		X
genes with unknown function
CpiED-A01	859	DQ401449	putative diapause upregulated unknown	n,x	no significant similarity		X
CpiED-C09	306	DQ401452	putative diapause upregulated unknown	x	46%, Anopheles gambiae, EAL40389		X
CpiED-D03	614	DQ401453	putative diapause upregulated unknown	x	66%, Anopheles gambiae, EAA10746		X
CpiLD-B02	312	DQ401450	late diapause upregulated unknown	x	42% Plasmodium yoelii yoelii, XP_727512		X
CpiLD-B11	841	DQ401451	late diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-D06	1,047	DQ401454	late diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-F01	445	DQ401455	late diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-F03	311	DQ401456	late diapause upregulated unknown	x	65%, Anopheles gambiae, XP_317882		X
CpiLD-F07	250	DQ401457	late diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-G10	519	DQ401458	putative diapause upregulated unknown	n	94%, Aedes aegypti, AY432235
CpiLD-H03	133	DQ401459	late diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-H05	398	DQ401460	putative diapause upregulated unknown	n,x	no significant similarity		X
CpiLD-M43	561	DQ401461	diapause upregulated unknown	n,x	no significant similarity	X	X