Table 2.

Diapause downregulated genes from Cx. pipiens, isolated by suppressive subtractive hybridization, and genes unchanged in diapause. Percent identities, organisms, and accession nos. were retrieved from GeneBank (http://www.ncbi.nlm.nih.gov/BLAST/). In the blast column, “n” and “x” indicate putative identities obtained through a nucleotide (BLASTn) and/or translation (BLASTx) query, respectively. Confirmation by northern blot is indicated by an “X” in nondiapause (18°C, long daylength; 7–10 days post adult eclosion) and early diapause (18°C, short daylength; 7–10 days post adult eclosion), and late diapause (18°C, short daylength; 56–59 days post adult eclosion).

clone	size	acc. no.	putative identity	blast	% identity, organism, accession no.
Diapause Downregulated Genes
food utilization
CpiED-A15	294	AY958427	chymotrypsin-like serine protease	x	45%, Aedes aegypti, AAX56968
CpiED-A34	264	AY958426	trypsin	n	98%, Culex pipiens pallens, AY034060
Genes Unchanged in Diapause
regulatory
CpiED-A18	292	DQ401462	putative poly A binding protein	n	91%, Aedes aegypti, AY431644
stress response
CpiED-A29	295	DQ401463	ubiquitin/ribosomal protein L40	n	94%, Aedes albopictus, AY826155
CpiLD-A10	333	DQ401464	cecropin	n	79%, Culex pipiens pipiens, AY189808
cytoskeletal
CpiED-B06	241	DQ401465	beta tubulin	n	89%, Drosophila melanogaster, NM_166356