Properties of viral vector systems

Vector	(1st & 2nd gen.) Adeno	(Gutless) Adeno	AAV	Lentivirus
Genome	dsDNA	dsDNA	ssDNA	RNA
Capacity (kb)	~ 8.3†	27–29	~4.8	~ 7.5 – 9†
Size (diameter)	~80 nm	~80nm	~20 nm	~80 nm
Integration	No	No	No/Yes*	Yes
Non-Dividing cell transduction	Yes	Yes	Yes	Yes
Duration of expression	Short	Long	Long	Long
CTL induction	Yes	Yes	No/Yes	No
Pre-existing immunity	Yes	Yes	No/Yes	No
Safety Issues	Inflammation	Cytotoxicity	Insertional mutagenesis?	Insertional mutagenesis

^†DNA payload size depends on the presence of accessory proteins

^*It is still unclear whether recombinant AAVs are capable of stable integration.