Table 1.

TIP39 analogs screened^a

−1	1	2	3	4	5	6	7	8	9	10	11	12	% Maximal PTH2-R activationb
	S	L	A	L	A	D	D	A	A	F	R	E	1E-06	1E-07	1E-08
						A							100	100	79
							A						100	100	100
										A			100	85	0
											A		100	100	100
				A									100	100	100
E													100	100	100
F													100	100	100
H													100	100	100
I													100	100	100
V													100	100	100
W													100	100	100
Y													100	100	100
			V										87	77	62
			W										69	63	45
			P										72	67	18
			L										41	52	51
			H										56	59	52
			F										60	46	41
			S										50	61	55
						L							3	0	0
						K							50	0	0
						S							54	1	0
						V							5	0	0
						E							82	65	71
						Q							65	26	0
						R							0	0	0
						A							100	0	0
						W							0	0	0
						M							73	0	0
						T							23	0	0
							H						23	6	1
						W	H						0	1	0
					Y	W	H						0	1	0
				P	Y	W	H						1	1	1
				H	Y	W	H						0	0	0
				H	Y	W	G						5	4	3
				H	Y	W	D						10	6	9
				H	Y	W	A						0	3	5
				H	Y	W	V						9	7	5
				H	Y	W	I						4	3	5
				H	Y	W	T						9	7	9
				H	Y	W	N						6	7	9

^aThe first 12 amino acid residues of TIP39 are indicated at the top. The analogs screened are indicated on subsequent rows. Either a single addition or substitution, or several substitutions, were incorporated as indicated. Standard single letter amino acid code.

^bThe percentage of the amount of cAMP accumulation produced by 1nM unmodified TIP39, in the presence of 10⁻⁶, 10⁻⁷ or 10⁻⁸ mutant peptide, in single screening experiments.