Fig 3. Inhibition of GSK-3β inhibits β-AR-stimulated increases in cytosolic cytochrome C levels.
ARVM were pretreated with SB216763 (20 μM; A) for 30 min or infected with adenoviruses expressing catalytically inactive GSK-3β (KM-GSK; B) for 42 h. The cells were then treated with ISO for 6 h. Cytosolic cytochrome C levels were measured using western blot analysis. Actin immunostaining indicates protein loading. *p<0.05 vs. control (CTL/GFP); n=3; #p<0.05 vs. ISO/GFP+ISO; n=3.