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. 2007 Jul;13(7):1071–1078. doi: 10.1261/rna.404407

FIGURE 1.

FIGURE 1.

(A) Editing and splicing of the GluR-B pre-mRNA from exon 11 through exon 16. (Gray bars) Exons, (lines) introns; not drawn to scale. (Arrows) The Q/R and R/G editing sites. Alternative splicing of exons 14 and 15 is indicated. (B) Editing/splicing reporter constructs used in the study. The pR2L minigene construct contains the natural GluR-B gene from exon 13 through exon 16. The GRG 988 construct contains a full-length intron 13, flanked by exons 13 and 14. In the GRG SS construct, exon 13 and the part of intron 13 required for R/G editing of GluR-B is fused to part of intron 1, the 3′-splice site, and exon 2 (MLex 2) from a modified adenovirus major late transcript. The GQR SS construct contains the R/G editing sequence fused to the adenovirus MLex2 3′-splice site. The β-globin reporter pAdCMV-glob is represented by exon 1 through exon 2 of the rabbit β-globin pre-mRNA. (Arrows) Positions of primers used for RT-PCR.