TABLE 1.

EFFECT OF ION TRANSPORT AGONISTS AND INHIBITORS ON SHORT CIRCUIT CURRENTS OF AIR–LIQUID INTERFACE CULTURES

Chemicals	Human Culture (ΔIsc, mean ± SEM)	Porcine Culture (ΔIsc, mean ± SEM)	Ferret Culture (ΔIsc, mean ± SEM)	Mouse Culture (ΔIsc, mean ± SEM)
Amiloride, 100 μM	−32.4 ± 3.7	−39.87 ± 3.0	−3.4 ± 0.6	−9.2 ± 0.7
DIDS, 100 μM	−7.1 ± 0.7	−5.35 ± 0.8	0.2 ± 0.4	−5.2 ± 0.6
IBMX/forskolin, 100 μM/10 μM	16.1 ± 0.9	35.2 ± 3.5	16.2 ± 1.8	21.3 ± 2.1
Bumetanide, 100 μM	−14.1 ± 1.3	−32.8 ± 3.4	−14.6 ± 2.0	−18.0 ± 1.6
Glibenclamide, 400 μM	−13.7 ± 0.9	−4.2 ± 0.6	−10.3 ± 1.3	11.5 ± 1.4
NPPB, 100 μM	−13.6 ± 0.6	−30.7 ± 3.1	−11.5 ± 1.3	−19.2 ± 1.8
CFTRinh 172, 20 μM	−11.7 ± 1.0	−26.6 ± 2.9	−2.2 ± 0.5	−16.0 ± 1.7
CFTRinh GlyH101, 10 μM	−14.7 ± 0.7	−30.1 ± 3.2	−12.2 ± 1.2	−2.9 ± 0.2

Definition of abbreviations: CFTR, cystic fibrosis transmembrane conductance regulator; DIDS, 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid; IBMX, 3-isobutyl-l-methylxanthine; I_sc, short circuit current; NPPB, 5-nitro-2-(3-phenylpropyl-amino)-benzoic acid.

Units of I_sc are in μA/cm².