TABLE 2.
EFFECT OF CHLORIDE CHANNELS BLOCKERS TO INHIBIT IBMX/FORSKOLIN-INDUCED CHLORIDE CURRENTS IN AIRWAY EPITHELIA
| Chemicals | Human Culture (%, mean ± SEM)* | Porcine Culture (%, mean ± SEM) | Ferret Culture (%, mean ± SEM) | Mouse Culture (%, mean ± SEM) |
|---|---|---|---|---|
| Bumetanide, 100 μM | 88.4 ± 3.2 | 93.2 ± 1.0 | 90.2 ± 10.0 | 84.4 ± 3.0 |
| Glibenclamide, 400 μM | 73.2 ± 5.3 | 10.3 ± 2.2 | 69.3 ± 3.4 | 69.5 ± 3.2 |
| NPPB, 100 μM | 85.4 ± 5.1 | 84.4 ± 4.7 | 67.8 ± 4.2 | 91.4 ± 2.5 |
| CFTRinh-172, 20 μM | 74.7 ± 5.1 | 55.6 ± 6.8 | 22.7 ± 3.9 | 75.3 ± 4.2 |
| CFTRinh-GlyH101, 10 μM | 91.1 ± 3.8 | 86.4 ± 1.5 | 78.6 ± 4.7 | 18.5 ± 2.0 |
Definition of abbreviations: CFTR, cystic fibrosis transmembrane conductance regulator; NPPB, 5-nitro-2-(3-phenylpropyl-amino)-benzoic acid.
*
The fraction of IBMX/forskolin-inhibited Isc in response to each inhibitor, relative to the total level of inhibition achieved after sequential addition of bumetanide, was used to calculate the % inhibition by each inhibitor.