Table 4.

Dosage effect of MAK-4 and MAK-5 on the growth of transformed Rat 6 cells

Treatment	Cells (× 106/60 mm plate) ± SD		% of survival
	Rat 6	R6/T24*	Rat 6	R6/T24
None (control)	0.94 ± 0.04	1.32 ± 0.04	100	100
MAK-4:
200 μg/ml	1.24 ± 0.12	1.52 ± 0.05	138	141
500 μg/ml	0.94 ± 0.08	1.27 ± 0.06	112	115
1000 μg/ml	0.82 ± 0.09	1.29 ± 0.07	91	119
MAK-5:
200 μg/ml	1.57 ± 0.09	1.77 ± 0.09	119	164
500 μg/ml	0.84 ± 0.08	1.07 ± 0.04	84	101
1000 μg/ml	0.58 ± 0.06	0.73 ± 0.01	66	79

R6/T24 is a permanent cell line transformed by an activated human c-H-ras oncogene. Both normal and H-ras transformed Rat 6 cells were seeded in triplicate at 10⁴ cells per 60 mm plate in D10CS. Next day, cultured were switched to D5FCS, or FCS plus herbal extracts at designated dosages for 5 days. At the end of the treatment, cells were trypsinized and counted by Coulter Counter. Cytotoxicity was expressed as percent of cell survival compared to the untreated control.