Table 1.

Erythroid progenitor numbers from the BM and spleens of wild-type and Lnk nullizygous mice

Progenitors by tissue type	Wild type*	Lnk-/-*	Fold increase (P)†	Fold increase per mouse
BM
Total cells per mouse, × 106	50 ± 12	63 ± 10	1.3 (.03)	1.3
BFU-e per 2 × 104 cells	10 ± 4	17 ± 5	1.7 (.01)	2.2
CFU-e per 2 × 105 cells	593 ± 100	517 ± 80	0.9 (.04)	1.1
Spleen
Total cells per mouse, × 106	150 ± 13	286 ± 68	1.9 (<.001)	1.9
BFU-e per 2 × 105 cells	30 ± 11	52 ± 18	1.7 (.03)	3.2
CFU-e per 1 × 106 cells	230 ± 45	546 ± 35	2.4 (.01)	4.6

Mice (8 to 12 weeks old) were used to isolate BM and spleen cells. Total BM and spleen cell numbers (mean ± SD) were enumerated in the presence of 3% acetic acid using a hemocytometer. An equal number of cells was plated in methylcellulose culture and enumerated for 2 days (CFU-e) and 10 days (BFU-e) after plating. The data were obtained from 6 to 8 mice from each genotype. Fold increase = Lnk^-/-/wild type. Fold increase per mouse (fifth column) = fold increase (fourth column) × fold increase in total cells.

^*Data shown are mean plus or minus SD.

^†P values determined by Student t test.