Figure 1.

LIBS-specific mAb-induced signaling by platelets lacking the β3 cytoplasmic domain. (A) Sequences of the αIIb and β3 cytoplasmic domains present in normal and VGTΔ724 platelets. (B) Aggregation traces of normal and VGTΔ724 platelets treated separately with the LIBS-specific mAbs D3 (30 μg/mL) and PT25-2 (30 μg/mL) in the presence of fibrinogen (Fg; 250 μg/mL). It is noteworthy that D3 and PT25-2 each induced shape change of VGTΔ724, but not the normal platelets. (C) Graph presentation of TxA₂ production as well as ATP and PF4 secretion. The error bars represent SD, n = 3. In some cases, the values of the SD were so small that the bars cannot be seen.